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KMID : 0545120010110061106
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 6 p.1106 ~ p.1110
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Electron Microscopic Evidence of Parasporal Crystal Inclusion Biogenesis in Bacillus sphaericus Strain 1593
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Lee Young-Ju
Lee Hyung-Hoan
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Abstract
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The parasporal biogenesis of crystal inclusion during the sporulation of Bacillus sphaericus strain 1593 was observed using transmission electron microscopy. The crystal biogenesis and sporulation process involved a sequence of events taking about 10 h. The sporulation processes were found to be similar to previous findings. The crystal biogenesis of B. sphaericus was initiated at the start of engulfment and nearly completed by the time of exosporium formation. The crystal formation was clearly associated with the outer forespore membrane from stages Ⅲ through Ⅵ, and the crystals grew from polypeptide-like chains originated from the outer forespore membrane. These observations are different from previous findings, which report no association with the forespore membrane. The crystals were located adjacent to the outer membrane of the spore until the release stage. The axes size of the bipyramidal crystal was approximately 0.25 ㎛×0.42 ㎛. During crystal biogenesis, the crystal development could be classified into four stages; initiation stage C1 (sporulation stage Ⅲ), growth stage C2 (sporulation Ⅲ to Ⅴ), envelopment and maturation C3 (sporulation Ⅴ to Ⅵ), and finally release stage C4 (sporulation Ⅶ).
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